The purpose of this sample was to carry forth the cause of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the experiment was also to understand the relationship between the change in absorbance with time and the enzyme reaction direct. To perform the experiment, the class was divided into groups. distributively group took on one of the factors affecting the enzyme. Before stem the actual experiment, we apply the mixing tables that were given to us to define up the test tubes with the appropriate substances. We followed the same procedure for individually experiment variable (1-5). We then rid the cell of hepatotoxic hydrogen peroxide (H2O2) so the cell would not contain damaged, as stated in our lab manual. For every observational variable, test tube 1 was always the control. Once the solutions were send we mixed them and poured them into the cuvette and used the spectrophotometer machine to run the enzyme and determine its rate and its absorbance. When the actual experiment was done we all shared the results and graphed them.
2) introduction
Before beginning the actual experiment we had to become long-familiar with a spectrometer machine and its functions.
It is basically an instrument used to measure the amount of light absorbed by a solution (Frankhauser, David B). As stated in our lab manual, the darker the solution, the greater its absorbance. The reason why we used this machine was to figure out the relationship between the change in absorbance with time and the enzyme reaction rate. The enzyme reaction rate was determined when plots of absorbance versus time were constructed. The slope of all(prenominal) plot was related to the reaction rate. After running a small experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as catalyst (Ophardt, Charles E.). By definition, a catalyst is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: Ordercustompaper.com
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